Osteogenic Differentiation Potential of Mesenchymal Stem Cells Using Single Cell Multiomic Analysis

Mesenchymal stem cells (MSC) are multipotent that can differentiate into multiple cell types, including osteoblasts, chondrocytes, and adipocytes. Osteoblast differentiation is reduced during osteoporosis development, resulting in bone formation. Further, MSC isolated from different donors possess distinct osteogenic capacity. In this study, we used single-cell multiomic analysis to profile the transcriptome epigenome of four healthy donors. Data were obtained ~1300 1600 for each donor. These clustered groups, indicating have chromatin accessible regulatory elements regulating gene expression. To investigate mechanism by which undergo differentiation, accessibility data multiome identify individual-specific enhancer–promoter pairs evaluated expression levels activities transcriptional regulators. The showed potential osteoblasts. donor 1 largest average motif activities, was most likely results our validation experiments consistent with bioinformatics prediction. We also tested enrichment genome-wide association study (GWAS) signals several musculoskeletal disease traits patient-specific regions identified data, osteoporosis, osteopenia, osteoarthritis. found osteoarthritis-associated variants only enriched 4. contrast, osteopenia least Since related density cells, these should be correlated MSC. summary, provides large-scale link their target genes relationships mesenchymal provide a deeper insight mechanism.